Comet Labs WN591 Bedienungsanleitung PDF herunterladen (Seite 18)

Help! There is a comet in my computer! 15

intensity from the total light intensity that we recorded. If we do not do this, we will

compromise the linear relationship between the amount of DNA on the slide and the grey

values on the image.

Figure 8. Grey values for the head, the tail and the background region of the model comet image.

Box segmentation is shown, with the head indicated in blue, the tail in red and the background in

yellow. The pixel column number eight, which is used as an example in the text, is outlined in dark

blue on the image and shown in blue numbers in the table. The number of each pixel column is

indicated in italic above the table.

head tail

background

12345678 9 10111213141516171819202122232425

41 41 41 41 41 41 41 41 41 41 41 41 41 41 41 41 41 51 41 51 41 41 41 41 41

41 41 41 41 41 41 41 46 41 46 41 46 41 41 46 41 41 41 41 41 41 46 41 41 41

41 51 41 41 41 41 41 41 41 41 41 41 41 41 41 41 41 41 41 41 41 41 41 41 41

46 41 41 41 41 41 41 41 41 41 41 41 46 41 41 41 41 46 41 46 41 41 41 41 41

sum

169

174

164

169

164

169

164

169

164

169

164

179

164

179

164

169

164

41 41 41 41 240 221 240 141 141 171 171 46 41 41 41 41 41 41 41 41 41 41 46 41 41

41 41 221 240 230 230 221 240 230 141 171 171 110 141 110 141 141 110 141 41 41 41 41 41 41

41 221 230 230 240 240 230 230 240 230 141 171 141 171 141 171 141 141 110 141 110 141 141 41 41

41 240 240 240 240 251 240 240 230 240 141 171 171 141 171 141 171 171 141 110 171 141 110 110 41

240 221 240 251 251 251 251 240 221 240 240 141 171 171 141 171 141 141 171 171 141 141 141 171 110

221 230 240 251 240 251 251 251 240 230 240 141 171 171 171 171 171 171 171 171 171 141 110 110 110

221 240 221 240 240 251 240 240 221 240 240 141 171 171 171 141 141 141 171 141 141 141 110 141 110

41 240 230 230 240 251 240 240 240 240 141 171 141 141 141 171 141 141 141 141 141 171 171 141 41

41 240 221 230 230 230 240 240 240 230 141 141 171 141 141 110 110 110 141 110 171 110 110 41 41

41 41 240 221 221 230 230 240 192 141 141 141 110 141 110 141 110 141 41 41 41 41 41 41 41

41 46 41 41 221 240 221 141 141 110 110 41 41 41 41 41 41 41 41 41 41 41 41 51 41

sum

1010

1801

2165

2215

2593

2646

2604

2443

2336

2213

1877

1476

1439

1471

1379

1440

1349

1310

1149

1210

1150

1062

929

658

Background

Head Tail

1 2 ... 13 14 15 16 17 18 19 20 21 22 23 ... 48 49

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