Comet Labs WN591 Bedienungsanleitung PDF herunterladen (Seite 2)

Help! There is a comet in my computer! i

Contents

Do you have a problem?...................................................................................... 1

1 Fluorescence microscopy............................................................................. 3

2 Detection of emitted light.............................................................................. 7

3 Measurement of the comet parameters ....................................................... 9

3.1 Grey values...............................................................................................................9

3.2 Segmentation..........................................................................................................10

3.3 Geometric calibration ............................................................................................11

3.4 Measurement of geometric parameters ...............................................................12

3.4.1 And I measured my comet at 400× magnification................................................13

3.5 Measurement of light intensity parameters .........................................................14

3.5.1 Integrated light intensity....................................................................................... 14

3.5.2 Percentage of DNA in the tail ..............................................................................17

3.5.3 Tail extent moment ..............................................................................................17

3.5.4 Comet profile .......................................................................................................18

3.5.5 Olive tail moment ................................................................................................. 19

4 Recorded image and displayed image....................................................... 21

4.1 Look-up table (LUT) ...............................................................................................21

4.2 Live image and grabbed image.............................................................................23

5 Image acquisition......................................................................................... 26

5.1 Histogram of image grey values ...........................................................................27

5.2 Image contrast and brightness .............................................................................27

5.3 Image saturation.....................................................................................................30

5.4 How can I evaluate the quality of my image? ......................................................32

5.5 Shading ...................................................................................................................35

5.6 How can I detect shading in my image? ..............................................................36

5.7 Why a 10-bit camera beats an 8-bit camera.........................................................38

5.8 Binning....................................................................................................................40

5.9 Which image does my software measure?..........................................................41

5.10 How do I get a good quality image out of my comet equipment? .....................42

6 Concluding remarks .................................................................................... 45

Notes ........................................................................................................ 46

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Comet Labs WN591 Bedienungsanleitung Seite 2